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Ana Karuza

Ana Karuza's picture
Nome: 
Ana
Cognome: 
Karuza
Sezione: 
OCE
Telefono: 
0402249742
Qualifica: 
Assegni di ricerca
Istruzione: 

Grammar school secondary degree

Prva hrvatska sušačka gimnazija u Rijeci ‘Andrija Mohorovičić’

20/06/1995, Rijeka (Croatia)

M. Sc. In Biological Sciences

University of Trieste (Italy)

20/07/2001

Dissertation title: Seasonal variations of growth and mortality of picoplanktonic popultations in the Gulf of Trieste and their relationships with the appearance of mucillage aggregates

Ph.D. in Biomonitoring methodologies of environmental alterations

University of Trieste (Italy)

20/04/2006

Dissertation title: Biogeochemical cycle of organic matter in marine coastal environment: role of viruses in controlling the development of bacterial populations

 

Esperienza: 

January 2016-current

OGS-affiliated

March 2006* – December 2015 

Post-doc in Microbial Ecology at OGS (Istituto Nazionale di Oceanografia e di Geofisica Sperimentale - OGS , former Biological Oceanography (BiO) Department, now Oceanography (OCE) Section

*interrupted twice: from December 2006 to April 2008 and from December 2008 to January 2010 for maternity leaves

January 2005 – December 2005: Post graduate fellowship by 'Consorzio per la Gestione del Laboratorio di Biologia marina' aimed to study marine microbial communities and their observations in epifluorescence microscopy;

January 2004 - January 2004: Post graduate fellowship by 'Consorzio per la Gestione del Laboratorio di Biologia marina' aimed to study marine microbial communities and their observations in epifluorescence microscopy;

January 2003 –  July 2003: Post graduate fellowship for the students of Alpe Adria Community awarded by University of Trieste and with the contribution of Friuli-Venezia Giulia Region aimed to the researches with topics resulting important for the geographic area Alpe Adria;

August 2003 – December 2003: Collaboration contract with Consorzio per la Gestione del Laboratorio di Biologia marina di Trieste.

July 2002 – December 2002: Collaboration contract with Consorzio per la Gestione del Laboratorio di Biologia marina di Trieste.

Capacità e interessi: 

Research activity in Marine Microbial Ecology

My research activity mainly focuses on planktonic marine microbes and on mechanisms that regulate their abundance, activity and divrsity in the sea. It investigates their pivotal role in biogeochemical fluxes, marine trophic webs, community structure and carbon partitioning in different marine ecosystems. During my apprenticeship my researh was centered on investigation of mortality rates of marine prokaryotes in the Gulf of Trieste due both to the grazing activity (by heterotrophic flagellates and micro-sized predators) and to the viral infection in a frame of experiments conducted on seasonal basis.

My post-graduate activity was mainly directed to the investigation of metabolic activity of heterotrophic prokaryotes. The results of reasearches conducted allowed to disentangle the bulk of these organisms into highly active, dead or dormant cells and their relationship with the trophic status of the environment, not only for the Gulf of Trieste on different temporal scales but also for the strong northern Adriatic trophic gradients in a frame of investigations conducted on seasonal basis.

The researches conducted during my Ph.D. internship were mainly focused on viral ecology. These studies deepened the knowledge about the role of virioplankton in microbial food webs and biogeochemical fluxes in the northern adriatic Sea by investigating viral abundance, production, their persistance and life strategies in the water column. Virus-mediated mortality on prokaryotic community as one of the main controls of their abundance and viral impact on prokaryotic biodiversity have been investigated as well. The role of viruses under forced environmental conditions (inorganic and organic substrate enrichment) was examined by means of laboratory experiments. The difficulty of finding reliable technique for the estimate of viral production rates, led us to the simultaneous assessment of three different methodological approaches.

Current investigations are mainly focussing on the impact of natural (i.e. extraordinary cold event, sporadic mucilage formations) and anthropoenic (side-effects of liquefied natural gas terminals, aquaculture, pollution, ocean acidification, wastewater treatmentr plants) stressors on marine trophic webs and water quality in the Adriatic, Ionian and Tyrrhenian seas. The risk assessment of potential leakage from CO2 storage sites as adverse effect of 'Carbon Capture and Storage' technology has been faced by studying the impact of increased CO2 concentrations on marine microbes and C pathways in natural analogues (Panarea, Italy).

Skills

  • Water sampling for biotic and abiotic parameters
  • Utilization of laboratory equipment, as centrifuges, microscopes (optical and epifluorescence), spectrophotometer, β-counter, peristaltic and depression filtration systems
  • Determination of microbial abundances in epifluorescence microscopy (viruses and prokaryotes)
  • Determination of highly active and non active or dead prokaryotic fractions at the given moment by means of dying (DAPI) or incubation methods (CTC, PI)
  • Determination of exoenzymatic prokaryotic activity by means of fluorogenic substrates
  • Estimate of prokaryotic secondary production by use of radiactive label (3H-TdR)
  • Estimate of viral production by use of different methodological approaches (dilution method, viral production in already infected prokaryotes, determination by use of radiactive label - 3H-TdR)
  • Quantification of lysogenic bacterial fraction
  • Estimate of growth and grazing rates by use of dilution approach
  • Bacteriological analyses (total coliforms, fecal coliforms, Streptococcus sp, Salmonella sp...) in water, sediments and edibile shellfish
  • Labile organic matter quantification (proteins, lipids and carbohydrates) by use of spectrophotometric methods
  • Windows (Office Package), graphics and impagination (Corel, Adobe-PageMaker e -Table, Golden Software-Grapher e –Surfer, CAD, Ocean Data View) and statistics (Statistica - StatSoft)
Major projects: 

ongoing             LTER - Long Term Ecological Research

2014 -                RITMARE - Ricerca Italiana per il Mare

2014 -                BALMAS - Ballast water management for Adriatic Sea protection

2013 - 2015      CCPWCP - Coastal Cities Pollution Water Control Project

2013                  PERSEUS-ADREX1 - Policy oriented marine Environmnetal Research for the Southern European Seas

2011 – 2013      ECO2 – Sub-seabed CO2 Storage: Impact on Marine Ecosystems

2011 – 2013      MEDSEA – Mediterranean Sea Acidification in a changing climate

2010 – 2012      RISCS – Research into Impacts and Safety in CO2 Storage

2010 - 2011       ENEL - Pre-injection offshore baseline survey

2008                  EMODNET – European Marine Observation and Data Network

2005 – 2007      ECOMADR – Ecology of Adriatic Sea Interreg III A Italy-Slovenia  

2004 – 2005      ISPESL – Identification of pathogenic opportunistic bacteria in bathing waters and in the areas used for shellfish farming funded by Italian Ministry of Health

2004 – 2005      CORILA – Consortium for Coordination of Research Activities Concerning the Venice Lagoon System II Research Programme aimed to investigate structure, dynamics and functional characteristics of biological communities in the Venice lagoon  (involved in WP2 - study of microbial loop in the Venice lagoon)

2002 – 2003      MAT - Processes of mucillage formation in the Adriatic and Thyrrenian Seas

2001 - 2002       In fondo al mar (At the bottom of the sea), joint project among Legambiente, WWF and Lega Pesca

2001 – 2006      INTERREG III - Monitoring project of the Northern Adriatic – Study of the trophic state and the anomalies of the northern Adriatic system

1999 – 2001      INTERREG II - Monitoring project of the Northern Adriatic – Study of the trophic state and the anomalies of the northern Adriatic

 

Lingue parlate: 
croato, italiano, inglese, tedesco, francese